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Author  |
Belyaev, I.Y.; Markova, E.; Hillert, L.; Malmgren, L.O.G.; Persson, B.R.R. |
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Title |
Microwaves from UMTS/GSM mobile phones induce long-lasting inhibition of 53BP1/gamma-H2AX DNA repair foci in human lymphocytes |
Type |
Journal Article |
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Year |
2009 |
Publication |
Bioelectromagnetics |
Abbreviated Journal |
Bioelectromagnetics |
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Volume |
30 |
Issue |
2 |
Pages |
129-141 |
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Keywords |
Adult; Case-Control Studies; *Cellular Phone; *DNA Repair; Female; Histones/*genetics; Humans; Intracellular Signaling Peptides and Proteins/*genetics; Lymphocytes/*radiation effects; Male; *Microwaves; Middle Aged |
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Abstract |
We have recently described frequency-dependent effects of mobile phone microwaves (MWs) of global system for mobile communication (GSM) on human lymphocytes from persons reporting hypersensitivity to electromagnetic fields and healthy persons. Contrary to GSM, universal global telecommunications system (UMTS) mobile phones emit wide-band MW signals. Hypothetically, UMTS MWs may result in higher biological effects compared to GSM signal because of eventual “effective” frequencies within the wideband. Here, we report for the first time that UMTS MWs affect chromatin and inhibit formation of DNA double-strand breaks co-localizing 53BP1/gamma-H2AX DNA repair foci in human lymphocytes from hypersensitive and healthy persons and confirm that effects of GSM MWs depend on carrier frequency. Remarkably, the effects of MWs on 53BP1/gamma-H2AX foci persisted up to 72 h following exposure of cells, even longer than the stress response following heat shock. The data are in line with the hypothesis that the type of signal, UMTS MWs, may have higher biological efficiency and possibly larger health risk effects compared to GSM radiation emissions. No significant differences in effects between groups of healthy and hypersensitive subjects were observed, except for the effects of UMTS MWs and GSM-915 MHz MWs on the formation of the DNA repair foci, which were different for hypersensitive (P < 0.02[53BP1]//0.01[gamma-H2AX]) but not for control subjects (P > 0.05). The non-parametric statistics used here did not indicate specificity of the differences revealed between the effects of GSM and UMTS MWs on cells from hypersensitive subjects and more data are needed to study the nature of these differences. |
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Address |
Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden. igor.belyaev@gmt.su.se |
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0197-8462 |
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PMID:18839414 |
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CBM.UAM @ ccobaleda @ |
Serial |
475 |
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Author |
Beneduci, A. |
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Title |
Evaluation of the potential in vitro antiproliferative effects of millimeter waves at some therapeutic frequencies on RPMI 7932 human skin malignant melanoma cells |
Type |
Journal Article |
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Year |
2009 |
Publication |
Cell Biochemistry and Biophysics |
Abbreviated Journal |
Cell Biochem Biophys |
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Volume |
55 |
Issue |
1 |
Pages |
25-32 |
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Keywords |
Cell Cycle/radiation effects; Cell Line, Tumor; Cell Proliferation/radiation effects; *Electromagnetic Fields; Humans; Melanoma/*radiotherapy; Microwaves; Skin Neoplasms/*radiotherapy |
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Abstract |
The potential antiproliferative effects of low power millimeter waves (MMWs) at 42.20 and 53.57 GHz on RPMI 7932 human skin melanoma cells were evaluated in vitro in order to ascertain if these two frequencies, comprised in the range of frequency used in millimeter wave therapy, would have a similar effect when applied in vivo to malignant melanoma tumours. Cells were exposed for 1 h exposure/day and to repeated exposure up to a total of four treatments. Plane wave incident power densities <1 mW/cm(2) were used in the MMWs-exposure experiments so that the radiations did not cause significant thermal effects. Numerical simulations of Petri dish reflectivity were made using the equations for the reflection coefficient of a multilayered system. Such analysis showed that the power densities transmitted into the aqueous samples were < or = 0.3 mW/cm(2). Two very important and general biological endpoints were evaluated in order to study the response of melanoma cells to these radiations, i.e. cell proliferation and cell cycle. Herein, we show that neither cell doubling time nor the cell cycle of RPMI 7932 cells was affected by the frequency of the GHz radiation and duration of the exposure, in the conditions above reported. |
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Department of Chemistry, University of Calabria, Via P. Bucci, Cubo 17/D, Arcavacata di Rende (CS), Italy. beneduci@unical.it |
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1085-9195 |
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PMID:19536459 |
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IT'IS @ evaj @ |
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286 |
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Author |
Beneduci, A.; Chidichimo, G.; De Rose, R.; Filippelli, L.; Straface, S.V.; Venuta, S. |
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Title |
Frequency and irradiation time-dependant antiproliferative effect of low-power millimeter waves on RPMI 7932 human melanoma cell line |
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Journal Article |
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Year |
2005 |
Publication |
Anticancer Research |
Abbreviated Journal |
Anticancer Res |
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Volume |
25 |
Issue |
2a |
Pages |
1023-1028 |
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Keywords |
Cell Line, Tumor; Cell Proliferation/radiation effects; *Electromagnetic Fields; Humans; Light; Melanoma/pathology/*radiotherapy; Microscopy; Microwaves |
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The biological effects produced by low power millimeter waves (MMW) were studied on the RPMI 7932 human melanoma cell line. Three different frequency-type irradiation modes were used: the 53.57-78.33 GHz wide-band frequency range, the 51.05 GHz and the 65.00 GHz monochromatic frequencies. In all three irradiation conditions, the radiation energy was low enough not to increase the temperature of the cellular samples. Three hours of radiation treatment, applied every day to the melanoma cell samples, were performed at each frequency exposure condition. The wide-band irradiation treatment effectively inhibited cell growth, while both the monochromatic irradiation treatments did not affect the growth trend of RPMI 7932 cells. A light microscopy analysis revealed that the low-intensity wide-band millimeter radiation induced significant morphological alterations on these cells. Furthermore, a histochemical study revealed the low proliferative state of the irradiated cells. This work provides further evidence of the antiproliferative effects on tumor cells induced by low power MMW in the 50-80 GHz frequency range of the electromagnetic spectrum. |
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Department of Chemistry, University of Calabria, 87036 Arcavacata di Rende (CS), Italy. beneduci@unical.it |
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0250-7005 |
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PMID:15868942 |
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Call Number |
IT'IS @ evaj @ |
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284 |
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Author |
Beneduci, A.; Chidichimo, G.; Tripepi, S.; Perrotta, E. |
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Title |
Transmission electron microscopy study of the effects produced by wide-band low-power millimeter waves on MCF-7 human breast cancer cells in culture |
Type |
Journal Article |
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Year |
2005 |
Publication |
Anticancer Research |
Abbreviated Journal |
Anticancer Res |
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Volume |
25 |
Issue |
2a |
Pages |
1009-1013 |
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Keywords |
Breast Neoplasms/*radiotherapy/*ultrastructure; Cell Line, Tumor/radiation effects; *Electromagnetic Fields; Humans; Microscopy, Electron, Transmission; Microwaves |
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Our previous work showed that low-power wide-band millimeter waves (MMW) inhibit the growth of the MCF-7 human breast carcinoma cell line, also causing a marked reduction of the density of microvilli at the apical membrane of the MCF-7 cells, as revealed by scanning electron microscopy. The aim of the present work was to investigate the ultrastructural changes induced by such electromagnetic radiations on this cell line. A transmission electron microscopy study was performed on MCF-7 cells irradiated under the same experimental conditions previously adopted. Transmission electron microscopy analysis revealed several ultrastructural features of the MMW-irradiated cells pertinent to cells subjected to sublethal injury. The antiproliferative effect of the millimeter radiation was confirmed. MMW, in the 52-78 GHz frequency range, act as stress factor on the cells that survive in a non-steady low-mitogenetic metabolic state. |
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Address |
Department of Chemistry, University of Calabria, Arcavacata di Rende (CS), Italy. beneduci@unical.it |
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0250-7005 |
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PMID:15868940 |
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Call Number |
IT'IS @ evaj @ |
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285 |
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Author |
Beneduci, A.; Chidichimo, G.; Tripepi, S.; Perrotta, E.; Cufone, F. |
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Title |
Antiproliferative effect of millimeter radiation on human erythromyeloid leukemia cell line K562 in culture: ultrastructural- and metabolic-induced changes |
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Journal Article |
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Year |
2007 |
Publication |
Bioelectrochemistry (Amsterdam, Netherlands) |
Abbreviated Journal |
Bioelectrochemistry |
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Volume |
70 |
Issue |
2 |
Pages |
214-220 |
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Keywords |
Cell Proliferation/*drug effects; Cell Survival/*radiation effects; Dose-Response Relationship, Radiation; Glucose/*metabolism; Glycolysis/*radiation effects; Humans; K562 Cells; Metabolic Clearance Rate/radiation effects; *Microwaves; Radiation Dosage |
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In the present study we compared the proliferation behavior, the ultrastructural morphology and the glycolitic metabolism of K562 cells irradiated by low-power wide-band millimeter waves, with those of sham-exposed K562 cells (control), maintained in the same culture conditions. The gigaHertz radiation treatments, performed between 53-78 10(9) Hz, induced a noticeable inhibition of the cell proliferation that could be related to relevant ultrastructural changes. Such effects brought the irradiated cell system to lose the homeostasis and to trigger defense/reparatory mechanisms in order to reestablish a new steady state. (13)C-Nuclear magnetic resonance data on the kinetic of glucose metabolism demonstrated that the irradiated cells enhanced the glycolitic aerobic pathway, indicating that such system need to produce an extra-bioenergy. Most of the ATP synthesized served probably to perform the above processes resulting in a significant decrease of the proliferation rate without significant cell death increment. |
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Department of Chemistry, University of Calabria, Via P. Bucci Cubo 17/D 87036 Arcavacata di Rende (CS), Italy. beneduci@unical.it <beneduci@unical.it> |
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1567-5394 |
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PMID:16959547 |
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Call Number |
IT'IS @ evaj @ |
Serial |
283 |
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