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Song, G., Qin, J., Yao, C., & Ju, Y. (2008). Effect of steep pulsed electric field on proliferation, viscoelasticity and adhesion of human hepatoma SMMC-7721 cells. Anticancer Res, 28(4b), 2245–2251.
Abstract: It has been proven that steep pulsed electric field (SPEF) can directly kill tumor cells and plays an important role in anticancer treatment. The biorheological mechanisms, however, that destroy tumor cells are almost unknown. To resolve this issue, here, an SPEF generator was used to assess the effects of high- and low-dose SPEF on the proliferation of human hepatoma SMMC-7721 cells by MTT assay, and on the viscoelasticity, adhesion of SMMC-7721 cells to endothelial cells by micropipette aspiration technique. Viability and proliferation of SPEF-treated SMMC-7721 cells were significantly inhibited. Cell cycle analysis indicated that SPEF arrested the cell cycle progression of SMMC-7721 cells at the G0/G1 transition to the S-phase. Viscoelastic data fitted by a standard linear solid model showed that viscoelasticity of SMMC-7721 cells changed after treatment with SPEF. Moreover, the adhesive force of low-dose SPEF-treated SMMC-7721 cells to endothelial cells markedly decreased compared to that of control cells. These results suggest that the suppressant effects of SPEF on the proliferation of SMMC-7721 cells appeared to be mediated, at least in part, through arresting cell cycle progression and altering the viscoelastic and adhesive properties of the cells, which provides a novel biorheological mechanism for the antitumor therapy of SPEF.
Keywords: Carcinoma, Hepatocellular/*pathology/*therapy; Cell Adhesion/radiation effects; Cell Growth Processes/radiation effects; Cell Line, Tumor; Cell Survival/radiation effects; Elasticity; Electric Stimulation Therapy/*methods; *Electromagnetic Fields; Endothelial Cells/cytology/radiation effects; Humans; Liver Neoplasms/*pathology/*therapy; Viscosity
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Sohrabi, M. - R., Tarjoman, T., Abadi, A., & Yavari, P. (2010). Living near overhead high voltage transmission power lines as a risk factor for childhood acute lymphoblastic leukemia: a case-control study. Asian Pac J Cancer Prev, 11(2), 423–427.
Abstract: This study aimed to investigate association of living near high voltage power lines with occurrence of childhood acute lymphoblastic leukemia (ALL). Through a case-control study 300 children aged 1-18 years with confirmed ALL were selected from all referral teaching centers for cancer. They interviewed for history of living near overhead high voltage power lines during at least past two years and compared with 300 controls which were individually matched for sex and approximate age. Logistic regression, chi square and paired t-tests were used for analysis when appropriate. The case group were living significantly closer to power lines (P<0.001). More than half of the cases were exposed to two or three types of power lines (P<0.02). Using logistic regression, odds ratio of 2.61 (95%CI: 1.73 to 3.94) calculated for less than 600 meters far from the nearest lines against more than 600 meters. This ratio estimated as 9.93 (95%CI: 3.47 to 28.5) for 123 KV, 10.78 (95%CI: 3.75 to 31) for 230 KV and 2.98 (95%CI: 0.93 to 9.54) for 400 KV lines. Odds of ALL decreased 0.61 for every 600 meters from the nearest power line. This study emphasizes that living close to high voltage power lines is a risk for ALL.
Keywords: Adolescent; Case-Control Studies; Child; Child, Preschool; Electric Power Supplies/*adverse effects; Electromagnetic Fields/*adverse effects; Environmental Exposure/*adverse effects; Female; Humans; Infant; Infant, Newborn; Leukemia, Radiation-Induced/*epidemiology; Male; Precursor Cell Lymphoblastic Leukemia-Lymphoma/*epidemiology/*etiology; Prognosis; Risk Assessment; Survival Rate
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Kuster, N. (2011). Increasing Bias towards False Negative Replications? BEMS NEWSLETTER, www.bems.org, (217), 1–5.
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Mullighan, C. G. (2011). Genomic profiling of B-progenitor acute lymphoblastic leukemia. Best Pract Res Clin Haematol, 24(4), 489–503.
Abstract: Childhood acute lymphoblastic leukemia (ALL) is comprised of multiple subtypes defined by recurring chromosomal alterations that are important events in leukemogenesis and are widely used in diagnosis and risk stratification, yet fail to fully explain the biology of this disease. In the last 5 years, genome-wide profiling of gene expression, structural DNA alterations and sequence variations has yielded important insights into the nature of submicroscopic genetic alterations that define novel subgroups of acute lymphoblastic leukemia and cooperate with known cytogenetic alterations in leukemogenesis. Importantly, several of these alterations are important determinants of risk of relapse and are potential targets for therapeutic intervention. Here, these advances and future directions in the genomic analysis of ALL are discussed.
Keywords: Animals; B-Cell-Specific Activator Protein/genetics; B-Lymphocytes/cytology/*metabolism; CREB-Binding Protein/genetics; Child; Child, Preschool; *Chromosome Aberrations; Fusion Proteins, bcr-abl/genetics; Gene Expression Profiling/*methods; Gene Expression Regulation, Neoplastic; Genomics/*methods; Humans; Ikaros Transcription Factor/genetics; Janus Kinase 1/genetics; Janus Kinase 2/genetics; Mice; *Mutation; Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*genetics/pathology; Receptors, Cytokine/genetics; Recurrence
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Doida, Y., Miller, M. W., Brayman, A. A., & Carstensen, E. L. (1996). A test of the hypothesis that ELF magnetic fields affect calcium uptake in rat thymocytes in vitro. Biochem Biophys Res Commun, 227(3), 834–838.
Abstract: The experiment's objective was to test the statistical hypothesis that a 60-min exposure of rat thymocytes in vitro to a unique combination of static and a.c. magnetic fields results in suppression of calcium influx triggered by concanavalin A (Con A). Con A (10 micrograms/ml) induced about a 50% increase in 45Ca2+ uptake relative to no Con A (control). Magnetic field exposures had no statistically significant effect on Con A-stimulated calcium uptake. The data did not support the hypothesis.
Keywords: Animals; Calcium/*metabolism; Concanavalin A; Ion Transport; *Magnetics; Male; Mitogens/pharmacology; Rats; Rats, Sprague-Dawley; Thymus Gland/cytology/drug effects/*metabolism
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