toggle visibility Search & Display Options

Select All    Deselect All
 |   | 
Details
   print

Warning: mysql_fetch_field() expects parameter 2 to be long, string given in /home/arimmora/public_html/refbase/includes/include.inc.php on line 309
  Record Links
Author (up) Benfante, R.; Antonini, R.A.; Kuster, N.; Schuderer, J.; Maercker, C.; Adlkofer, F.; Clementi, F.; Fornasari, D. url  doi
openurl 
  Title The expression of PHOX2A, PHOX2B and of their target gene dopamine-beta-hydroxylase (DbetaH) is not modified by exposure to extremely-low-frequency electromagnetic field (ELF-EMF) in a human neuronal model Type Journal Article
  Year 2008 Publication Toxicology in Vitro : an International Journal Published in Association With BIBRA Abbreviated Journal Toxicol In Vitro  
  Volume 22 Issue 6 Pages 1489-1495  
  Keywords Cell Line, Tumor; Dopamine beta-Hydroxylase/radiation effects; Electromagnetic Fields/*adverse effects; Gene Expression/*radiation effects; Homeodomain Proteins/*radiation effects; Humans; Neuroblastoma/metabolism; Time Factors; Transcription Factors/*radiation effects; Transcription, Genetic/radiation effects; Tretinoin/pharmacology  
  Abstract The homeodomain transcription factors PHOX2A and PHOX2B are vital for development of the autonomic nervous system. Their spatial and temporal expression at the neural crest is instrumental in determining neuronal precursor fate, and by regulating DbetaH expression, the enzyme catalysing noradrenaline synthesis from dopamine, they also play a role in determination of noradrenergic phenotype. Disturbing this finely regulated process leads to disruption of autonomic development and autonomic dysfunction syndromes such as DbetaH deficiency. As it had previously been shown that the catecholamine system is responsive to ELF-EMF, and as this has also been linked to various pathologies and to certain types of cancer, we wondered whether exposure to this type of radiation could affect the expression of PHOX2A, PHOX2B and DbetaH, also during differentiation triggered by retinoic acid. To investigate this possibility we exposed the human SH-SY5Y neuroblastoma cell line to 50 Hz power-line magnetic field at various flux densities and for various exposure times. We measured gene expression in exposed cells compared to control cells and also investigated any changes at protein level. Using our exposure protocol, we found no changes at either transcript or protein level of these important components of the autonomic nervous system and catecholaminergic system.  
  Address Department of Pharmacology, School of Medicine, University of Milan, via Vanvitelli 32, Milan, Italy  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0887-2333 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:18572378 Approved no  
  Call Number ITIS @ murbach @ Serial 164  
Permanent link to this record
Select All    Deselect All
 |   | 
Details
   print

Save Citations:
Export Records: