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Author (up) Saito, A.; Takayama, Y.; Moriguchi, H.; Kotani, K.; Jimbo, Y. url  doi
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  Title Developmental effects of low frequency magnetic fields on P19-derived neuronal cells Type Journal Article
  Year 2009 Publication Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference Abbreviated Journal Conf Proc IEEE Eng Med Biol Soc  
  Volume 2009 Issue Pages 5942-5945  
  Keywords Cell Differentiation/*radiation effects; Cell Line; Dose-Response Relationship, Radiation; Electromagnetic Fields; Humans; Nerve Net/*cytology/*physiology/radiation effects; Neurons/*cytology/*physiology/radiation effects; Radiation Dosage  
  Abstract Modulation of pluripotent stem cell differentiation by several environmental factors, such as physical stimulation, is important theme in tissue engineering. In this study, we report the effects of extremely low frequency magnetic fields (ELF-MFs) exposure (1 mT or 10 mT, 50 Hz, sinusoidal) on the neuronal differentiation process of P19 embryonal carcinoma cells (P19 cells). Here, during induction of differentiation, the ELF-MFs exposed to embryoid bodies (EBs). After neuronal differentiation, the effects of ELF-MFs were evaluated by morphological analysis, immunochemical analysis (MAP2, GFAP), and the developmental neuronal network activities recorded by the micro-electrode arrays (MEAs). As a result, the percentage of MAP2 positive cells and the spike frequencies were increased by 10 mT ELF-MF, and then the percentage of GFAP positive cells were reduced. However, these effects were not seen in 1 mT exposed cells. Therefore, these results suggested that the intensity of a magnetic field was important for affecting a characteristic of neuronal differentiation and a functional neuronal network property.  
  Address Department of Human and Engineered Environment Studies, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan. d097620@h.k.u-tokyo.ac.jp  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1557-170X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:19965063 Approved no  
  Call Number CBM.UAM @ ccobaleda @ Serial 520  
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