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Author  |
Razavi, S.; Salimi, M.; Shahbazi-Gahrouei, D.; Karbasi, S.; Kermani, S. |
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Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells |
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2014 |
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Advanced biomedical research |
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3 |
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25-25 |
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BACKGROUND: Extremely low-frequency electromagnetic fields (ELF-EMF) can effect on biological systems and alters some cell functions like proliferation rate. Therefore, we aimed to attempt the evaluation effect of ELF-EMF on the growth of human adipose derived stem cells (hADSCs). MATERIALS AND METHODS: ELF-EMF was generated by a system including autotransformer, multi-meter, solenoid coils, teslameter and its probe. We assessed the effect of ELF-EMF with intensity of 0.5 and 1 mT and power line frequency 50 Hz on the survival of hADSCs for 20 and 40 min/day for 7 days by MTT assay. One-way analysis of variance was used to assessment the significant differences in groups. RESULTS: ELF-EMF has maximum effect with intensity of 1 mT for 20 min/day on proliferation of hADSCs. The survival and proliferation effect (PE) in all exposure groups were significantly higher than that in sham groups (P < 0.05) except in group of 1 mT and 40 min/day. CONCLUSION: Our results show that between 0.5 m and 1 mT ELF-EMF could be enhances survival and PE of hADSCs conserving the duration of exposure. |
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WP5 In vitro |
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UNIBAS @ david.schuermann @ |
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560 |
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