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Author  |
Zhu, H.; Wang, J.; Cui, J.; Fan, X. |
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Title |
Effects of extremely low frequency electromagnetic fields on human fetal scleral fibroblasts |
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Journal Article |
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2014 |
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Toxicology and industrial health |
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August |
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collagen type i; extremely low frequency electromagnetic; fibroblast; field; matrix metalloproteinase-2; sclera; transforming growth factor beta-2 |
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Abstract |
This study investigated the effects of extremely low frequency electromagnetic fields (ELF-EMFs) on human fetal scleral fibroblasts (HFSFs). HFSFs were subjected to 50 Hz artificial ELF-EMFs generated by Helmholtz coils with 0.1, 0.2, 0.5, and 1.0 mT field intensities for 6 to 48 h. The viability and factors involved in scleral structuring of HFSFs were determined. The growth rate of HFSFs significantly decreased after only 24 h of exposure to ELF-EMFs (0.2 mT). The messenger RNA (mRNA) expression of collagen type I (COL1A1) decreased and expression of matrix metalloproteinase-2 (MMP-2) increased significantly. There was a decrease in tissue inhibitor of MMP-2 mRNA levels between treated and control cells only at the 1.0 mT intensity level. Transforming growth factor beta-2 mRNA increased in exposed cells, and, simultaneously, fibroblast growth factor-2 mRNA levels decreased. The protein expressions of COL1A1 and MMP-2 were also significantly altered subsequent to exposure (p < 0.05). This study shows that ELF-EMFs had biological effects on HFSFs and could cause abnormality in scleral collagen. |
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WP5 In vitro |
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UNIBAS @ david.schuermann @ |
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629 |
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