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Chen, C.; Ma, Q.; Liu, C.; Deng, P.; Zhu, G.; Zhang, L.; He, M.; Lu, Y.; Duan, W.; Pei, L.; Li, M.; Yu, Z.; Zhou, Z. |
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Title |
Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells |
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Journal Article |
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Year |
2014 |
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Scientific reports |
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4 |
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30 |
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5103-5103 |
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A radiofrequency electromagnetic field (RF-EMF) of 1800 MHz is widely used in mobile communications. However, the effects of RF-EMFs on cell biology are unclear. Embryonic neural stem cells (eNSCs) play a critical role in brain development. Thus, detecting the effects of RF-EMF on eNSCs is important for exploring the effects of RF-EMF on brain development. Here, we exposed eNSCs to 1800 MHz RF-EMF at specific absorption rate (SAR) values of 1, 2, and 4 W/kg for 1, 2, and 3 days. We found that 1800 MHz RF-EMF exposure did not influence eNSC apoptosis, proliferation, cell cycle or the mRNA expressions of related genes. RF-EMF exposure also did not alter the ratio of eNSC differentiated neurons and astrocytes. However, neurite outgrowth of eNSC differentiated neurons was inhibited after 4 W/kg RF-EMF exposure for 3 days. Additionally, the mRNA and protein expression of the proneural genes Ngn1 and NeuroD, which are crucial for neurite outgrowth, were decreased after RF-EMF exposure. The expression of their inhibitor Hes1 was upregulated by RF-EMF exposure. These results together suggested that 1800 MHz RF-EMF exposure impairs neurite outgrowth of eNSCs. More attention should be given to the potential adverse effects of RF-EMF exposure on brain development. |
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UNIBAS @ david.schuermann @ |
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558 |
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Jin, Y.B.; Choi, S.-H.; Lee, J.S.; Kim, J.-K.; Lee, J.-W.; Hong, S.-C.; Myung, S.H.; Lee, Y.-S. |
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Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression |
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Journal Article |
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2014 |
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Radiation and environmental biophysics |
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53 |
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1 |
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93-101 |
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combined; extremely low frequency á; tail moment for dna; treatment á comet olive |
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The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H(2)O(2) (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H(2)O(2), or c-Myc activation. |
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UNIBAS @ david.schuermann @ |
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559 |
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Razavi, S.; Salimi, M.; Shahbazi-Gahrouei, D.; Karbasi, S.; Kermani, S. |
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Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells |
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Journal Article |
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2014 |
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Advanced biomedical research |
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3 |
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25-25 |
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BACKGROUND: Extremely low-frequency electromagnetic fields (ELF-EMF) can effect on biological systems and alters some cell functions like proliferation rate. Therefore, we aimed to attempt the evaluation effect of ELF-EMF on the growth of human adipose derived stem cells (hADSCs). MATERIALS AND METHODS: ELF-EMF was generated by a system including autotransformer, multi-meter, solenoid coils, teslameter and its probe. We assessed the effect of ELF-EMF with intensity of 0.5 and 1 mT and power line frequency 50 Hz on the survival of hADSCs for 20 and 40 min/day for 7 days by MTT assay. One-way analysis of variance was used to assessment the significant differences in groups. RESULTS: ELF-EMF has maximum effect with intensity of 1 mT for 20 min/day on proliferation of hADSCs. The survival and proliferation effect (PE) in all exposure groups were significantly higher than that in sham groups (P < 0.05) except in group of 1 mT and 40 min/day. CONCLUSION: Our results show that between 0.5 m and 1 mT ELF-EMF could be enhances survival and PE of hADSCs conserving the duration of exposure. |
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UNIBAS @ david.schuermann @ |
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560 |
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Speit, G.; Gminski, R.; Tauber, R. |
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Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in HL-60 cells are not reproducible |
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Journal Article |
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2013 |
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Mutation research |
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755 |
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2 |
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163-166 |
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Comet Assay; HL-60 Cells; HL-60 Cells: radiation effects; Humans; Micronucleus Tests; Multicenter Studies as Topic; Radio Waves; Radio Waves: adverse effects; Reproducibility of Results; Research Design |
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Conflicting results have been published regarding the induction of genotoxic effects by exposure to radiofrequency electromagnetic fields (RF-EMF). Various results indicating a genotoxic potential of RF-EMF were reported by the collaborative EU-funded REFLEX (Risk Evaluation of Potential Environmental Hazards From Low Energy Electromagnetic Field Exposure Using Sensitive in vitro Methods) project. There has been a long-lasting scientific debate about the reliability of the reported results and an attempt to reproduce parts of the results obtained with human fibroblasts failed. Another part of the REFLEX study was performed in Berlin with the human lymphoblastoid cell line HL-60; genotoxic effects of RF-EMF were measured by means of the comet assay and the micronucleus test. The plausibility and reliability of these results were also questioned. In order to contribute to a clarification of the biological significance of the reported findings, a repeat study was performed, involving scientists of the original study. Comet-assay experiments and micronucleus tests were performed under the same experimental conditions that had led to genotoxic effects in the REFLEX study. Here we report that the attempts to reproduce the induction of genotoxic effects by RF-EMF in HL-60 cells failed. No genotoxic effects of RF-EMF were measured in the repeat experiments. We could not find an explanation for the conflicting results. However, the negative repeat experiments suggest that the biological significance of genotoxic effects of RF-EMF reported by the REFLEX study should be re-assessed. |
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Elsevier B.V. |
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UNIBAS @ david.schuermann @ |
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561 |
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Sheikh, A.Q.; Taghian, T.; Hemingway, B.; Cho, H.; Kogan, A.B.; Narmoneva, D.A. |
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Title |
Regulation of endothelial MAPK/ERK signalling and capillary morphogenesis by low-amplitude electric field |
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Journal Article |
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Year |
2013 |
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Journal of the Royal Society, Interface / the Royal Society |
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10 |
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78 |
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20120548-20120548 |
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Animals; Apoptosis; Calcium; Calcium: metabolism; Capillaries; Capillaries: cytology; Capillaries: growth & development; Cell Proliferation; Cells; Cultured; Electromagnetic Fields; Extracellular Signal-Regulated MAP Kinases; Extracellular Signal-Regulated MAP Kinases: metabo; Gene Expression Regulation; MAP Kinase Kinase 4; MAP Kinase Kinase 4: metabolism; MAP Kinase Signaling System; Mice; Morphogenesis; Neovascularization; Phosphatidylinositol 3-Kinases; Phosphatidylinositol 3-Kinases: metabolism; Phosphorylation; Physiologic; Proto-Oncogene Proteins c-raf; Proto-Oncogene Proteins c-raf: metabolism; Time Factors; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor A: biosynthesis |
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Low-amplitude electric field (EF) is an important component of wound-healing response and can promote vascular tissue repair; however, the mechanisms of action on endothelium remain unclear. We hypothesized that physiological amplitude EF regulates angiogenic response of microvascular endothelial cells via activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. A custom set-up allowed non-thermal application of EF of high (7.5 GHz) and low (60 Hz) frequency. Cell responses following up to 24 h of EF exposure, including proliferation and apoptosis, capillary morphogenesis, vascular endothelial growth factor (VEGF) expression and MAPK pathways activation were quantified. A db/db mouse model of diabetic wound healing was used for in vivo validation. High-frequency EF enhanced capillary morphogenesis, VEGF release, MEK-cRaf complex formation, MEK and ERK phosphorylation, whereas no MAPK/JNK and MAPK/p38 pathways activation was observed. The endothelial response to EF did not require VEGF binding to VEGFR2 receptor. EF-induced MEK phosphorylation was reversed in the presence of MEK and Ca(2+) inhibitors, reduced by endothelial nitric oxide synthase inhibition, and did not depend on PI3K pathway activation. The results provide evidence for a novel intracellular mechanism for EF regulation of endothelial angiogenic response via frequency-sensitive MAPK/ERK pathway activation, with important implications for EF-based therapies for vascular tissue regeneration. |
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UNIBAS @ david.schuermann @ |
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562 |
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